Fig. 3.

Alignment of HEV-C104 sequences reveal several nucleotide differences in the region of the putative FTD21 HEV amplicon. The week 16 throat swab sequence (kvv585-16-TS) was aligned to other strains of HEV-C104; enteroviruses group A, B, C, and D consensus sequences; and 2 HRV strains in the region targeted by the in-house PCR system (nucleotide positions 360-556 in HEV-C104 AK11, represented by *). A dashed line denotes the position of the predicted amplicon of the FTD21 kit. Regions where forward primer, reverse primer, and the probe from the in-house HEV PCR system anneal are color coded (regions 360-374, 544-566, 449-471, respectively) as well as putative regions of mismatches responsible for impaired detection by the FTD21 kit (regions 430-433 and 482-488).