Table 1.
Recombination events in the S1 gene of a novel recombinant IBV variant (TH/IBV/2014/CU-179 and TH/IBV/2016/CU-92) detected by RDP4 software.
Virus name | Breakpoints |
Major parent1 (similarity) | Minor parent2 (similarity) | Detection methods (P-value)3 | |
---|---|---|---|---|---|
Beginning | Ending | ||||
TH/IBV/2014/CU-179 | 891 | 1,608 | TH/IBV/2016/CU-123 (96%) | TH/IBV/2016/CU-99 (100%) | RDP, GENECONV, BootScan, MaxChi, Chimaera, SiScan, 3Seq (7.668 × 10−44, 8.756 × 10−37, 1.422 × 10−35, 2.063 × 10−23, 1.880 × 10−23, 1.216 × 10−29, 1.004 × 10−57) |
TH/IBV/2016/CU-92 | 918 | 1,633 | TH/IBV/2016/CU-123 (96.2%) | TH/IBV/2016/CU-99 (99.9%) | RDP, GENECONV, BootScan, MaxChi, Chimaera, SiScan, 3Seq (7.668 × 10−44, 8.756 × 10−37, 1.422 × 10−35, 2.063 × 10−23, 1.880 × 10−23, 1.216 × 10−29, 1.004 × 10−57) |
Major parent is the gene sequence of parent providing the larger part of the recombinant virus's sequence.
Minor parent is the gene sequence of parent providing the smaller part of the recombinant virus's sequence.
All recombination events were detected by all 7 detection methods and the recombination events were accepted only when they were detected by 5 or more methods implemented in the RDP4, with a P-value lower than 10−14.