Fig. 3.

Accumulation of viral coat proteins (CPs) and RNA in oat protoplasts inoculated with premature termination and initiation codon mutants of the CP gene. Oat protoplasts were inoculated with capped transcripts of wild type clone pOBDV, premature termination mutants GH1-7 and EF2-2, and initiation codon mutants IJ4-7 (minor CP), and KL1-3 and KL2-5 (major CP) and incubated for 24 h. Methods for detection of viral protein and RNA were as described in the legend for Fig. 2. The locations of major CP (Maj. CP), minor CP (Min. CP), genomic (gRNA), and subgenomic RNA (sgRNA) on the blots are shown. Partially purified OBDV (OBDV prep) was included on the western blot for size comparison.