Fig. 4.

Accumulation of viral coat proteins (CPs) and RNA in oat protoplasts inoculated with putative polymerase/CP cleavage site mutants. Oat protoplasts were inoculated with capped transcripts of wild type clone pOBDV, putative cleavage site mutant MN6-1, and double mutant IJMN-9 (minor CP initiation codon mutation combined with putative cleavage site mutation; IJMN-9A and B represent two different inoculations) and incubated for 24 h. Methods for detection of viral protein and RNA were as described in the legend for Fig. 2. The locations of major CP (Maj. CP), minor CP (Min. CP), genomic (gRNA), and subgenomic RNA (sgRNA) on the blots are shown. Partially purified OBDV (OBDV prep) was included on the western blot for size comparison.