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Capsase-12 is activated earlier than caspase-8 and caspase-3. Vero E6 cell lysates following TULV infection on the indicated day p.i. were collected for immunoblotting and analyzed with antibodies against caspase-12, caspase-8, and caspase-3, respectively. The cleavage of caspase-12 took place on the fourth day p.i., while caspase-8 and caspase-3 were activated on the fifth day p.i.
