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. 2004 Sep 11;343(4):819–831. doi: 10.1016/j.jmb.2004.08.071

Figure 4.

Figure 4

Oligomerisation states and N-RNA binding activities of full-length P and P173-297. A, Full-length P and P173-297 were incubated with different concentrations of the cross-linking agent EGS as indicated and analysed by 12% Tris–glycine SDS-PAGE for intact P and SDS-16% PAGE for P173-297. B, N-RNA templates isolated from infected BSR cells were incubated with full-length P and P173-297. After centrifugation of the N–RNA-P mixture through sucrose cushions, proteins present in the supernatants (S) and pellets (P) were analysed by SDS-16% PAGE.