Table 2.

List of primers used for the detection of TSV, YHV, EF-1α and β-actin gene by SYBR Green RT-PCR

Virus/Control gene	Primer	SYBR Green Primer sequence (5′–3′)	%GC	Tma	Amplicon size (bp)
TSV	112F	For: CTGTTTGTAACACTACCTCCTGGAATT	40	52	50
	162R	Rev: TGATACAACAACCAGTGGAGGACTAA	42	51
YHV	141F	For: CGTCCCGGCAATTGTGAT	55	45	65
	206R	Rev: CCAGTGACGTTCGATGCAATA	47	47
EF-1α	123F	For: TCGCCGAACTGCTGACCAAGA	57	51	55
	123R	Rev: CCGGCTTCCAGTTCCTTACC	60	51
β-actin	178F	For: GGTCGGTATGGGTCAGAAGGA	57	51	50
	228R	Rev: TTGCTTTGGGCCTCATCAC	52	46

^aAt 50 mM Na⁺.