Table 3.

The cycle threshold (C_T) values of replicate assays for TSV and YHV plasmid DNA dilutions

Plasmid standards	CT valuesa			Mean	SDb	CVc
	Expt. 1	Expt. 2	Expt. 3
TSV plasmid copy no.
2.42×106	19.383	19.117	19.650	19.383	0.267	1.376
2.42×105	23.403	22.703	22.237	22.781	0.587	2.578
2.42×104	27.130	26.840	26.103	26.691	0.529	1.983
2.42×103	30.737	30.557	30.040	30.444	0.362	1.188
2.42×102	34.443	33.777	34.153	34.124	0.334	0.980
2.42×101	35.747	38.220	37.237	37.068	1.245	3.359
12.1	36.703	38.543	38.580	37.942	1.073	2.828
6.05	37.017	39.447	39.727	38.730	1.490	3.848
3.03	39.580	39.713	39.747	39.680	0.088	0.222
1.51	40.000	40.000	40.000	40.000	0.000	0.000
YHV plasmid copy no.
2.12×106	18.503	18.503	18.797	18.601	0.169	0.910
2.12×105	21.520	21.520	21.707	21.582	0.108	0.499
2.12×104	24.640	24.757	24.873	24.757	0.117	0.471
212×103	28.323	28.263	28.263	28.283	0.035	0.122
2.12×102	32.083	31.750	32.197	32.010	0.232	0.725
2.12×101	34.727	35.557	35.443	35.242	0.450	1.277
10.6	36.443	36.470	36.467	36.460	0.015	0.040
5.3	38.033	38.100	38.813	38.316	0.432	1.128
2.6	37.383	38.417	39.510	38.437	1.063	2.767
1.33	39.113	38.557	39.163	38.944	0.337	0.865

^aThe C_T value is the average of 3 replicates of SYBR Green PCR run of that experiment.

^bStandard deviation.

^cCoefficient of variation.