Table 4.

Inter-experimental variabilities in the cycle threshold (C_T) values of TSV and YHV samples and their corresponding internal controls (EF-1α and β-actin) using cDNA as template

Virus	cDNA samples	CT valuesa			Mean	SDb	CVc
		Expt. 1	Expt. 2	Expt. 3
TSV	P. vannamei (Kona) stock
	K1	28.440	30.490	29.280	29.403	1.031	3.505
	K2	28.475	29.210	29.120	28.935	0.401	1.386
	K3	33.355	33.940	33.210	33.502	0.386	1.154
	K4	24.135	26.005	24.965	25.035	0.937	3.743
	K5	33.775	33.930	33.585	33.763	0.173	0.512
	K6	26.900	28.395	28.160	27.818	0.804	2.890
	K7	35.365	36.465	35.460	35.763	0.610	1.704
	K8	27.795	28.535	28.485	28.272	0.414	1.463
	K9	26.535	26.600	26.350	26.495	0.130	0.490
	K10	28.985	31.415	30.725	30.375	1.252	4.123
	EF-1α
	K1	23.655	25.495	24.565	24.572	0.920	3.744
	K2	21.860	23.105	23.045	22.670	0.702	3.097
	K3	23.275	24.290	23.850	23.805	0.509	2.138
	K4	21.535	23.765	22.615	22.638	1.115	4.926
	K5	20.950	21.690	21.865	21.502	0.486	2.259
	K6	21.220	22.695	22.285	22.067	0.761	3.450
	K7	22.135	23.640	23.265	23.013	0.783	3.404
	K8	21.270	22.280	22.345	21.965	0.603	2.744
	K9	21.960	22.940	22.870	22.590	0.547	2.420
	K10	24.125	25.505	25.440	25.023	0.779	3.112
	β-Actin
	K1	24.130	23.715	23.875	23.907	0.209	0.876
	K2	22.440	22.255	22.355	22.350	0.093	0.414
	K3	26.090	25.930	25.845	25.955	0.124	0.479
	K4	27.590	21.065	20.260	22.972	4.020	3.350
	K5	23.870	24.035	23.880	23.928	0.093	0.387
	K6	19.760	19.380	19.170	19.437	0.299	1.539
	K7	21.400	21.090	21.290	21.260	0.157	0.739
	K8	20.115	19.440	20.075	19.877	0.379	1.905
	K9	20.765	20.270	20.495	20.510	0.248	1.208
	K10	21.385	NT	20.975	21.180	0.290	1.369
YHV	Super Shrimp (SS) P.stylirostris
	SS1	20.635	21.330	20.865	20.943	0.354	1.691
	SS2	28.780	27.110	27.305	27.732	0.913	3.293
	SS3	24.725	24.015	23.270	24.003	0.728	3.031
	SS4	20.535	20.780	20.810	20.708	0.151	0.728
	SS5	26.575	25.500	26.770	26.282	0.684	2.602
	SS6	26.300	27.455	25.530	26.428	0.969	3.666
	SS7	22.430	22.905	22.075	22.470	0.416	1.853
	SS8	24.805	25.270	25.020	25.032	0.233	0.930
	SS9	26.135	26.170	25.805	26.037	0.201	0.773
	SS10	25.505	25.095	25.040	25.213	0.254	1.008
	EF-1α
	SS1	22.395	22.330	22.580	22.435	0.130	0.578
	SS2	26.415	25.065	26.405	25.962	0.777	2.991
	SS3	21.650	21.650	21.360	21.553	0.167	0.777
	SS4	21.165	21.510	21.510	21.395	0.199	0.931
	SS5	29.275	30.040	30.105	29.807	0.462	1.549
	SS6	28.195	26.240	27.270	27.235	0.978	3.591
	SS7	25.020	24.220	25.150	24.797	0.504	2.031
	SS8	24.800	25.125	25.215	25.047	0.218	0.872
	SS9	24.865	24.030	24.920	24.605	0.499	2.027
	SS10	24.150	25.865	25.040	25.018	0.858	3.428
	β-Actin
	SS1	22.055	23.180	NT	22.618	0.795	3.517
	SS2	30.430	28.460	28.865	29.252	1.040	3.557
	SS3	20.325	20.935	20.655	20.638	0.305	1.479
	SS4	19.855	20.700	20.700	20.418	0.488	2.389
	SS5	29.020	29.255	29.940	29.405	0.360	1.225
	SS6	27.390	28.185	26.210	27.262	0.994	3.645
	SS7	25.345	26.055	25.085	25.495	0.502	1.969
	SS8	26.750	27.290	27.020	27.020	0.270	0.999
	SS9	25.250	25.730	24.710	25.230	0.510	2.023
	SS10	26.325	27.930	27.790	27.348	0.889	3.251

NT, not tested.

^aFor each sample, the C_T value is the average of 2–3 replicates of SYBR Green PCR run of that day.

^bStandard deviation.

^cCoefficient of variation.