Fig. 6.

Reconstruction to determine the effect of variable SKI-1/S1P cleavage efficiency on envelope glycoprotein fusogenic potential. Vero 76 cells were transfected with 4 μg DNA containing either the wild-type GP-C plasmid (wt), a 1:3 mixture of the wt and cd-JGPC plasmids, respectively, or a 1:9 mixture of the same plasmids. Parallel cultures were metabolically labeled and immunoprecipitated, and PNGase F-treated polypeptides were analyzed as described in Fig. 2, Fig. 3 (A) or subjected to the recombinant vaccinia virus-based fusion assay as described in Fig. 5 (B).