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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: J Mol Cell Cardiol. 2019 Sep 3;136:72–84. doi: 10.1016/j.yjmcc.2019.08.013

Figure 5. PNM have faster fission/fusion rates than IFM.

Figure 5

(A, D) Confocal images of cardiomyocytes transfected with Ad-DsRed before and after photobleaching (PB) the ROIs at 540 nm (scale bar: 10 μm, scale bar of enlarged images: 2 μm). (B, C) FRAP time course and rate for PN and IF mitochondria. (D,E) Exemplar ROI where an existing mitochondrion appears to enter the ROI during FRAP period. (F-H) FRAP of DsRed signal after photobleaching with and without Drp1 inhibition by Mdivi-1 (n=7 cells).