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. 2016 Apr 20;34(30):3478–3492. doi: 10.1016/j.vaccine.2016.04.020

Table 3.

Summary of virus isolation, PCR testing and viral sequencing from nasal swabs of BRD treated calves recently vaccinated with MLV vaccine – Group 2.

IDa DOFb Temp Location
 BoHV-1
 BVDV
Feedlot Vaccine Gel PCR Culture Sequence q-PCR q-PCR results Gel PCR Culture Sequence qRT-PCR qTR-PCR results
1911-95806 13 105.3 Kansas Vax 2 Negative Positive MLV Vax 1-2 , Not detected Negative Negative ,32.78 Positive
1883-97138 18 105.6 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1849-98671 29 106.5 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1858-96614 25 104.9 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1900-99874 13 104.7 Kansas Vax 2 Negative Negative ,35.07c Suspect Negative Negative ,36.87 Suspect
194-95779 13 105.2 Kansas Vax 2 Negative Negative ,35.35 Suspect Negative Negative , Not detected
1876-92378 22 105.4 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1910-99793 12 105.3 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1900-99545 13 105.3 Kansas Vax 2 Negative Negative ,35.83 Suspect Negative Negative , Not detected
1938-97756 5 104.8 Kansas Vax 2 Positive Negative ,31.67 Positive Negative Negative ,38.60 Suspect
1927-94130 6 104.6 Kansas Vax 2 Negative Negative ,36.17 Suspect Negative Negative , Not detected
1845-92438 29 104.6 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1924-95955 10 106.3 Kansas Vax 2 Positive Positive MLV Vax 1-2 ,13.62 Positive Positive Positive BVDV1b NCP ,24.75 Positive
1916-92958 11 104.7 Kansas Vax 2 Negative Negative ,33.27 Positive Negative Negative , Not detected
1911-95770 12 107.0 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1936-93345 5 104.8 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1911-95812 12 105.2 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
1884-95524 15 105.8 Kansas Vax 2 Negative Positive MLV Vax 1-2 , Not detected Negative Negative , Not detected
6160-7679 16 105.1 Kansas Vax 2 Negative Negative , Not detected Negative Negative ,35.60 Suspect
6165-7158 14 102.0 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected
7405-4273 21 105.0 Kansas Vax 2 Positive Negative , Not detected Negative Negative , Not detected
7750-8609 NAd 105.8 Kansas Vax 2 Negative Negative , Not detected Negative Negative ,37.05 Suspect
7405-4594 NA 106.6 Kansas Vax 2 Negative Negative ,28.79 Positive Negative Negative , Not detected
1101-13108157 NA NA Kansas Vax 4 Positive Negative , Not detected Positive Positive MLV BVDV1a C24V ,27.77 Positive
6121-8302 23 105.6 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected
6160-7607 17 105.5 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected
6127-6579 21 105.0 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected
7750-8644 11 106.2 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected
7742-7633 18 106.1 Kansas Vax 3 Negative Negative , Not detected Negative Negative ,37.96 Suspect
7738-6225 NA 107.2 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected
7749-6569 11 107.0 Kansas Vax 3 Negative Negative , Not detected Negative Positive MLV BVDV1a Singer ,32.13 Positive
7415-4710 18 106.0 Kansas Vax 3 Positive Positive MLV Vax 1-2 ,27.52 Positive Negative Positive BVDV1b NCP ,28.97 Positive
7437-5511 8 105.0 Kansas Vax 3 Positive Positive MLV Vax 1-2 ,33.70 Positive Negative Negative , Not detected
1101-13108197 NA NA Kansas Vax 3 Positive Negative , Not detected Positive Positive BVDV‘1a Not Vax ,32.17 Positive
7925-563 NA NA Texas Vax 3 Positive Negative , Not detected Negative Positive BVDV1a Not Vax ,30.99 Positive
852-890 14 104.4 Texas Vax 3 Negative Positive Field strain , Not detected Negative Negative ,36.11 Suspect
876-459 12 106.0 Texas Vax 3 Positive Positive Field strain , Not detected Negative Positive BVDV 1b NCP ,28.95 Positive
850-995 20 104.4 Texas Vax 3 Negative Negative , Not detected Negative Negative , Not detected
836-648 25 104.5 Texas Vax 3 Negative Positive Field strain , Not detected Negative Negative , Not detected
IDa DOFb Temp Location
 BoCV
 BRSV
 PI3V
Feedlot Vaccine Gel PCR Culture qRT-PCR qTR-PCR results Gel PCR Culture qRT-PCR qTR-PCR results Gel PCR Culture Sequence
1911-95806 13 105.3 Kansas Vax 2 Negative Negative ,30.88 Positive Negative Negative , Not detected Negative Culture PI3V B
1883-97138 18 105.6 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Positive
1849-98671 29 106.5 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1858-96614 25 104.9 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1900-99874 13 104.7 Kansas Vax 2 Negative Negative ,35.33 Suspect Positive Negative ,23.39 Positive Negative Negative
194-95779 13 105.2 Kansas Vax 2 Negative Negative ,34.67 Positive Negative Negative , Not detected Negative Negative
1876-92378 22 105.4 Kansas Vax 2 Negative Negative ,34.85 Positive Negative Negative , Not detected Negative Negative
1910-99793 12 105.3 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1900-99545 13 105.3 Kansas Vax 2 Negative Positive ,31.69 Positive Positive Negative ,31.41 Positive Negative Negative
1938-97756 5 104.8 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1927-94130 6 104.6 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1845-92438 29 104.6 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1924-95955 10 106.3 Kansas Vax 2 Negative Positive ,32.46 Positive Positive Negative ,29.15 Positive Negative Negative
1916-92958 11 104.7 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1911-95770 12 107.0 Kansas Vax 2 Negative Negative ,35.77 Suspect Negative Negative , Not detected Negative Negative
1936-93345 5 104.8 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1911-95812 12 105.2 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
1884-95524 15 105.8 Kansas Vax 2 Negative Positive ,34.95 Positive Negative Negative , Not detected Negative Negative PI3V B
6160-7679 16 105.1 Kansas Vax 2 Negative Negative ,35.90 Suspect Negative Negative , Not detected Negative Positive
6165-7158 14 102.0 Kansas Vax 2 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
7405-4273 21 105.0 Kansas Vax 2 Negative Negative ,37.02 Suspect Negative Negative , Not detected Negative Negative
7750-8609 NAd 105.8 Kansas Vax 2 Positive Positive ,22.54 Positive Negative Negative ,38.74 Suspect Negative Negative
7405-4594 NA 106.6 Kansas Vax 2 Negative Negative ,36.31 Suspect Negative Negative , Not detected Negative Negative
1101-13108157 NA NA Kansas Vax 4 Negative Negative ,35.34 Suspect Positive Negative ,28.80 Positive Negative Negative
6121-8302 23 105.6 Kansas Vax 3 Negative Negative ,35.33 Suspect Negative Negative , Not detected Negative Negative
6160-7607 17 105.5 Kansas Vax 3 Negative Negative ,35.33 Suspect Negative Negative ,33.71 Positive Negative Negative
6127-6579 21 105.0 Kansas Vax 3 Negative Negative ,36.00 Suspect Negative Negative , Not detected Negative Negative
7750-8644 11 106.2 Kansas Vax 3 Negative Negative ,36.07 Suspect Negative Negative , Not detected Negative Negative
7742-7633 18 106.1 Kansas Vax 3 Negative Negative ,31.68 Positive Positive Negative ,32.34 Positive Negative Negative
7738-6225 NA 107.2 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
7749-6569 11 107.0 Kansas Vax 3 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
7415-4710 18 106.0 Kansas Vax 3 Negative Negative ,31.45 Positive Positive Negative ,29.13 Positive Negative Negative
7437-5511 8 105.0 Kansas Vax 3 Positive Negative ,23.54 Positive Negative Negative , Not detected Negative Negative
1101-13108197 NA NA Kansas Vax 3 Negative Negative ,35.96 Suspect Negative Negative , Not detected Negative Negative
7925-563 NA NA Texas Vax 3 Positive Positive ,17.00 Positive Negative Negative , Not detected Negative Negative
852-890 14 104.4 Texas Vax 3 Negative Negative ,33.96 Positive Negative Negative , Not detected Negative Negative
876-459 12 106.0 Texas Vax 3 Negative Negative ,27.40 Positive Negative Negative , Not detected Negative Negative
850-995 20 104.4 Texas Vax 3 Negative Negative ,37.24 Suspect Negative Negative , Not detected Negative Negative
836-648 25 104.5 Texas Vax 3 Negative Negative , Not detected Negative Negative , Not detected Negative Negative
a

The animal identification.

b

Days on feed.

c

Ct values.

d

Not available.