. 2016 Apr 20;34(30):3478–3492. doi: 10.1016/j.vaccine.2016.04.020

Table 4.

Summary of virus isolation, PCR testing and viral sequencing from nasal swabs of BRD treated calves recently vaccinated with MLV vaccine – Group 3.

ID^a	DOF^b	Temp	Location		BoHV-1					BVDV
			Feedlots	Vaccine	Gel PCR	Culture	Sequence	RT-PCR CT	RT-PCR results	Gel PCR	Culture	Sequence	RT-PCR CT	RT-PCR results
7785-0108	6	104.0	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
7777-8061	20	104.7	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
7777-8064	20	105.2	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
2966-3447	5	107.1	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
2948-2100	21	106.6	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,37.86	Suspect
2966-3329	5	105.2	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
2948-2163	21	104.4	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
2955-1368	5	105.8	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
7491-5976	13	105.2	Kansas	Vax 1	Negative	Positive	MLV Vax 1-2	,33.65^c	Positive	Negative	Negative		,	Not detected
2968-2015	4	106.3	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
2966-3484	4	105.2	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
6222-7854	17	106.8	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
6229-6864	11	105.4	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
6241-8936	7	105.0	Kansas	Vax 3	Negative	Negative		,	Not detected	Positive	Positive	bvdv1b ncp	,29.13	Positive
4947-810	22	105.0	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
5661-4243	7	105.1	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
5651-8319	15	106.9	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,36.82	Suspect
5654-5797	12	104.4	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
5632-5696	20	104.0	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
4950-778	20	105.5	Kansas	Vax 5	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
4967-333	11	105.3	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
4950-825	20	104.5	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
4953-438	19	104.3	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected
4952-762	20	104.3	Kansas	Vax 3	Negative	Positive	Field strain	,	Not detected	Negative	Negative		,	Not detected
4952-831	20	105.3	Kansas	Vax 3	Negative	Negative		,	Not detected	Positive	Positive	mlv bvdv1a singer	,37.10	Suspect
2948-2122	21	106.2	Kansas	Vax 3	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected

ID^a	DOF^b	Temp	Location		BoCV				BRSV				PI3V
			Feedlots	Vaccine	Gel PCR	Culture	RT-PCR CT	RT-PCR Results	Gel PCR	Culture	RT-PCR CT	RT-PCR results	Gel PCR	Culture	Sequence
7785-0108	6	104.0	Kansas	Vax 3	Positive	Positive	,22.92	Positive	Negative	Negative	,	Not detected	Negative	Negative
7777-8061	20	104.7	Kansas	Vax 3	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Negative
7777-8064	20	105.2	Kansas	Vax 3	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Negative
2966-3447	5	107.1	Kansas	Vax 3	Positive	Positive	,17.10	Positive	Negative	Negative	,	Not detected	Negative	Negative
2948-2100	21	106.6	Kansas	Vax 3	Negative	Negative	,37.38	Suspect	Negative	Negative	,	Not detected	Negative	Negative
2966-3329	5	105.2	Kansas	Vax 3	Positive	Positive	,16.16	Positive	Negative	Negative	,	Not detected	Negative	Negative
2948-2163	21	104.4	Kansas	Vax 3	Negative	Negative	,34.36	Positive	Negative	Negative	,	Not detected	Negative	Negative
2955-1368	5	105.8	Kansas	Vax 3	Negative	Negative	,37.43	Suspect	Negative	Negative	,	Not detected	Negative	Negative
7491-5976	13	105.2	Kansas	Vax 1	Negative	Negative	,37.43	Suspect	Negative	Negative	,	Not detected	Negative	Negative
2968-2015	4	106.3	Kansas	Vax 3	Positive	Positive	,21.57	Positive	Positive	Negative	,35.89	Suspect	Negative	Negative
2966-3484	4	105.2	Kansas	Vax 3	Positive	Negative	,26.42	Positive	Negative	Negative	,	Not detected	Negative	Negative
6222-7854	17	106.8	Kansas	Vax 3	Negative	Negative	,29.35	Positive	Negative	Negative	,	Not detected	Negative	Negative
6229-6864	11	105.4	Kansas	Vax 3	Negative	Negative	,37.83	Suspect	Negative	Negative	,	Not detected	Negative	Negative
6241-8936	7	105.0	Kansas	Vax 3	Negative	Negative	,35.55	Suspect	Negative	Negative	,29.18	Positive	Negative	Negative
4947-810	22	105.0	Kansas	Vax 3	Negative	Negative	,29.51	Positive	Negative	Negative	,	Not detected	Negative	Negative
5661-4243	7	105.1	Kansas	Vax 3	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Negative
5651-8319	15	106.9	Kansas	Vax 3	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Negative
5654-5797	12	104.4	Kansas	Vax 3	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Positive	PI3V C
5632-5696	20	104.0	Kansas	Vax 3	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Negative
4950-778	20	105.5	Kansas	Vax 5	Negative	Positive	,	Not detected	Negative	Negative	,	Not detected	Negative	Positive	PI3V C
4967-333	11	105.3	Kansas	Vax 3	Positive	Negative	,28.67	Positive	Positive	Negative	,	Not detected	Negative	Negative
4950-825	20	104.5	Kansas	Vax 3	Negative	Negative		Not detected	Negative	Negative	,	Not detected	Negative	Positive	PI3V C
4953-438	19	104.3	Kansas	Vax 3	Negative	Negative	,34.03	Positive	Negative	Negative	,	Not detected	Positive	Positive	PI3V C
4952-762	20	104.3	Kansas	Vax 3	Negative	Negative	,33.12	Positive	Positive	Negative	,25.81	Positive	Negative	Negative
4952-831	20	105.3	Kansas	Vax 3	Negative	Positive	,32.43	Positive	Negative	Negative	,	Not detected	Negative	Positive	PI3V C
2948-2122	21	106.2	Kansas	Vax 3	Negative	Positive	,37.33	Suspect	Negative	Negative	,	Not detected	Negative	Negative

The animal identification.

Days on feed.

Ct values.