Table 5.

Summary of virus isolation, PCR testing and viral sequencing from tissues of BRD treated calves recently vaccinated with MLV vaccine – necropsy cases from Groups 2 and 3.

IDa	DOFb	Location		Pathology	Tissue	BoHV-1					BVDV					BoCV				BRSV				PI3V
		Feedlots	Vaccine	Diagnosis	Homogenate	Gel PCR	Culture	Sequence	q-PCR	q-PCR results	Gel PCR	Culture	Sequence	qRT-PCR	qRT-PCR results	Gel PCR	Culture	qRT-PCR	qRT-PCR results	Gel PCR	Culture	qRT-PCR	qRT-PCR results	Gel PCR	Culture	Sequence
1839-9847	35	Kansas	Vax 2	Severe, fibrinosuppurative and necrotizing tracheitis, compatible with IBR	Trachea	Positive	Positive	Field strain	,23.74c	Positive	Negative	Negative		,	Not detected	Negative	Negative	,33.81	Positive	Negative	Negative	,	Not detected	Negative	Negative
7379-3498	NAd	Texas	Vax 3	Gross lesions-tracheitis	Lung	Positive	Positive	MLV Vax 1-2	,29.08	Positive	Negative	Positive	MLV BVDV1a NADL	,39.07	Suspect
				Fresh tissues only	Trachea	Positive	Positive	MLV Vax 1-2	,25.20	Positive	Negative	Negative				Negative	Negative	,37.43	Suspect	Negative	Negative	,	Not detected	Negative	Negative
5360-9054	NA	Kansas	Vax 3	Acute to subacute, severe intersitital pneumonia, compatible with AIP	Lung	Negative	Negative		,	Not detected	Negative	Positive	Did not band on PCR	,	Not detected	Negative	Negative	,35.26	Suspect	Negative	Negative	,	Not detected	Negative	Negative
7399-3408	39	Kansas	Vax 1	Severe fibrinosuppurative tracheitis, compatible with IBR	Trachea	Negative	Negative		,	Not detected	Positive	Positive	Twe bands on PCR	,22.39	Positive	Negative	Positve	,	Not detected	Negative	Negative	,	Not detected	Negative	Positive	PI3V C
				Acute to severe fibrinosuppurative bronchopneumonia and pleuritis												Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative
6242-7997	7	Kansas	Vax 3	Severe, acute fibrinosuppurative pleuropneumona with lobular necrosis and hemorrhage compatible with pulmonary Mannheimiosis. Histophilus somni and Mannheimia haemolytica isolated e	Lung	Negative	Positive	Field strain	,	Not detected	Positive	Positive	MLV BVDV1a Singer	,29.08	Positive
7469	5	Kansas	Vax 3	No history, fresh tissues only	Lung	Negative	Negative		,	Not detected	Negative	Negative		,	Not detected	Negative	Positive	,28.04	Positive	Negative	Negative	,	Not detected	Negative
6250-7254	3	Kansas	Vax 3	Fibrinous pneumonia	Lung	Negative	Positive	Field strain	,	Not detected	Positive	Positive	MLV BVDV1a Singer	,24.96	Positive	Negative	Negative	,	Not detected	Negative	Negative	,	Not detected	Negative	Positive	PI3V B
																Negative	Negative	,	Not detected	Positive	Negative	,22.74	Positive	Negative	Negative

^aThe animal identification.

^bDays on feed.

^cCt values.

^dNot available.

^eTesting at Kansas State University Veterinary Diagnostic Laboratory, Manhattan, Kansas.