Figure 4.

Sequences of Recombinant RNAs

Aligned sequences of the 5′ fragment and the 3′ fragment are shown below and above a recombinant sequence, respectively. Only partial sequences are shown; other parts of the recombinants perfectly match sequences of the respective fragments. Foreign extensions of the fragments are shown in boldface letters, and positions where the extensions differ are shown in lowercase letters. Foreign inserts in the recombinant sequences are shown in white letters on a black background. Numbers in parentheses indicate the number of clones whose sequences are identical.

(A) Sequences generated upon preincubation during 40 min prior to colony growth of a mixture of 10¹⁰ molecules each of the 5′ and 3′ fragments of RQ135⁻¹(−) RNA in the absence of Mg²⁺.

(B) Sequences generated upon preincubation of the fragments in the presence of 10 mM MgCl₂.

(C) Sequence of a clone isolated from products of the control RT-PCR amplification of the same mixture upon its preincubation in the presence of Mg²⁺.