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. 2001 Apr 11;88(4):503–513. doi: 10.1016/S0092-8674(00)81890-5

Figure 6.

Figure 6

Replication of Recombinant RNAs

Polyacrylamide gel electrophoresis pattern of recombinant RNAs stained with toluidine blue. Numbered letters above the gel refer to the type of recombinant sequence (Figure 4), numbers at the bottom refer to the clone number.

(A) Samples containing 1–2 μg of transcripts synthesized by T7 RNA polymerase from the cloned recombinant plasmids digested at site SmaI.

(B) Products of 10 min Qβ replicase reactions initiated with 1/400 of the amount of RNA applied to gel A. The faster migrating band is a double-stranded RNA, the slower migrating bands correspond to single strands and partial duplexes. Lane marked “0” contains material from the reaction where no RNA was added.