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. 2016 Apr 28;234:137–141. doi: 10.1016/j.jviromet.2016.04.018

Fig. 2.

Fig. 2

Specificity of reverse transcription-PCR assays. PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with EZ-VISION® THREE 6× loading buffer. (a) PCR products of primary amplification with primer pair F1/UR and using cDNA as templates; (b) PCR products of secondary amplification with primer pair F2/UR using 10−2 diluted first round PCR products as template; (c) PCR products of secondary amplification with primer pair F3/UR and using 10−2 diluted first round PCR products as templates. M: 100 bp ladder DNA marker (PHENIX Research Products, NC, USA); Lane 1: PEDV strain PC177; Lane 2: PEDV strain PC22A; Lane 3: PEDV strain Iowa106; Lane 4: a mixture of PC177, PC22A and Iowa106 with 1:1:1 ratio; Line 5: a mixture of PC177, PC22A and Iowa106 with 10:1:1 ratio; Line 6: a mixture of PC177, PC22A and Iowa106 with 1:10:1 ratio; Line 7: a mixture of PC177, PC22A and Iowa106 with 1:1:10 ratio; Line 8: porcine transmissible gastroenteritis virus (TGEV); Line 9: porcine deltacoronavirus (PDCoV).