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. 2016 Apr 28;234:137–141. doi: 10.1016/j.jviromet.2016.04.018

Fig. 3.

Fig. 3

Sensitivity of the primary PCR assays with the individual primer set. The sensitivity of each PCR assay was determined using 10-fold serial dilutions (from original cell culture supernatants to 1:105 in RNase-free water) of each strain. PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with EZ-VISION® THREE 6× loading buffer. M: 100 bp ladder DNA marker (PHENIX Research Products, NC, USA); Lines 1–6: 100–10−5 diluted virus stocks. (a) PCR products of PC177 strain with primer pair F1/UR; (b) PCR products of PC22A strain with primer pair F2/UR; (c) PCR products of Iowa106 strain with primer pair F3/UR.