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Differences in growth morphology between normal intestinal epithelial (IPEC-J2) (a, c) and tumorigenic cells (Caco-2) (b, d) and growth type (a,b — simple monolayers on plastic; c,d — 3D growth). Magnification 100×. Both cell lines develop different surface morphology depending on the growth matrix (monolayer — 3D). 3D growth allows the cells to polarise with distinct apical and basolateral parts, mimicking the in vivo situation.
