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. 2016 May 31;126:77–83. doi: 10.1016/j.pep.2016.05.018

Table 2.

Summary of S25-229 protein yields.

Steps Protein (mg) Purity (%) Step yield (%) Overall yield (%)
IBsa 7.5 ≥80 100 100
Solubilization 7.1 ≥80 95 95
Refolding 6.3 ≥80 89 83
CE chromatography 1.5 ≥95 23 20
a

75 mg of inclusion body proteins were obtained from 2.28 g wet cells harvested from 0.5 liter E. coli culture. An aliquot of 7.5 mg IBs were used as a starting material for purification.