Table 2.

Overall performance of the multiplex.

Assessment		adenovirus	astrovirus	rotavirus	sapovirus
EDL	Single	10−3+/−	10−3	10−5	10−6
	Multiplex	10−4+/−	10−5+/−	10−5	10−5

	R2	Slope	R2	Slope	R2	Slope	R2	Slope
Linearity and efficiency ranges	0.99–1.0	−3.1 → 55	0.99	−3.2 →51	0.99	−3.11 → 27	0.99	−3.1 → 25

Repeatability and reproducibility		Mean Ct	CV	Mean Ct	CV	Mean Ct	CV	Mean Ct	CV
Intra-assay	S3	22.3	0.00479	18.14	0.00529	18.87	0.00903	18.51	0.00656
	S2	27.83	0.00829	24.2	0.00788	24.66	0.01525	24.16	0.00612
	S1	33.93	0.01847	34.13	0.01037	34.6	0.00887	30.75	0.01106
Inter-assay	S3	22.08	0.02397	17.9	0.00582	18.88	0.01497	18.17	0.01221
	S2	27.28	0.12473	24.2	0.01727	24.64	0.03103	24.07	0.01248
	S1	34.29	0.01735	32.14	0.14854	31.8	0.02164	30.67	0.01364

EDL: endpoint detection limit; R2: reaction efficiency; C_V: co-efficient of variation.