Table 1.
Transformation of E. coli and A. tumefaciens with viral genome-encoding plasmid and infectivity of clones.
| Virus | Rep |
E. coli transformation |
A. tumefaciens transformation |
|||||
|---|---|---|---|---|---|---|---|---|
| Colonies | PCR (positive/tested) | Sequencing (correct/tested) | Colonies | PCR (positive/tested) | Sequencing (correct/tested) | Inoculation (symptomatic/tested) | ||
| PRSV | 1 | 0 | 106 | 17/20 | 3/3 | 30/31 | ||
| 2 | 2 | 2/2 | 0/2 | 52 | 12/14 | 2/3 | 30/32 | |
| 3 | 0 | 98 | 20/24 | 2/2 | 30/30 | |||
| PLDMV | 1 | 1 | 1/1 | 0/1 | 197 | 14/14 | 4/4 | 20/22 |
| 2 | 2 | 1/2 | 0/2 | 103 | 10/12 | 2/2 | 20/20 | |
| 3 | 0 | 54 | 8/12 | 2/3 | 21/22 | |||