Table 2.
Primers used in SOE PCRs to construct CfaB-epitope fusion genes in the study.
| Primer | Sequence (5’-3’) | Amplified region |
|---|---|---|
| CfaB-F | CGGGCTAGCGTAGAGAAAAATATT | upstream of CfaB gene, NheI site underlined |
| CfaB-R | TTACGGCCGGGATCCCAAAGTCAT | downstream of CfaB gene, EagI site underlined |
| FedF-ep1-F | AGCACTACTCGCACTAGAATTGATTTTTTTAGTTGCATCGTTTGT | forward and reverse primers to insert FedF epitope 1 into CfaB gene |
| FedF-ep1-R | TCTAGTGCGAGTAGTGCTCAAGTCGATACACCACAGCTTACAGAT | |
| FedF-ep2-F | AGTGTTTGTCTTCCCTGTGCCAAGTTTTTTAGTTGCATCGTTTGT | forward and reverse primers to insert FedF epitope 2 into CfaB gene |
| FedF-ep2-R | ACAGGGAAGACAAACACTACCCAAATGGATACACCACAGCTTACA | |
| FedF-ep3-F | GCATGTCAAAGTTCCTGATGAACTAGGAATTTTTTTAGTTGCATC | forward and reverse primers to insert FedF epitope 3 into CfaB gene |
| FedF-ep3-R | TCAGGAACTTTGACATGCCAGGCTGGAACTGATACACCACAGCTT | |
| FedF-ep4-F | CTGTTGCCCCCACTGAGATTCATTTTTTTTAGTTGCATCGTTTGT | forward and reverse primers to insert FedF epitope 4 into CfaB gene |
| FedF-ep4-R | TCTCAGTGGGGGCAACAGTCACAAGATACACCACAGCTTACAGAT | |
| FedF-ep5-F | GGAAGAAAGGGGATATGTCTGAGCTTTTTTAGTTGCATC | forward and reverse primers to insert FedF epitope 5 into CfaB gene |
| FedF-ep5-R | ACATATCCCCTTTCTTCCGGTGATGATACACCACAGCTT | |
| FedF-ep6-F | TGAAGGCATATCATTTTGGTTGGGTTTTTTAGTTGCATCGTTTGT | forward and reverse primers to insert FedF epitope 6 into CfaB gene |
| FedF-ep6-R | CAAAATGATATGCCTTCATCTAATGATACACCACAGCTTACAGAT | |
| FedF-ep7-F | CCACGAGCCTGTTGCATCGGGCTGTTTTTTAGTTGCATCGTTTGT | forward and reverse primers to insert FedF epitope 7 into CfaB gene |
| FedF-ep7-R | GATGCAACAGGCTCGTGGTATGATGATACACCACAGCTTACAGAT |