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Comparison of Expression Levels of Perforin and Granzyme B Genes in Expanded NK Cells (eNKC) at the Presence of Autologous Feeder Layer and OKT3 and Isolated NK Cells (iNKC) after Stimulation with Target HCC Cells at Different E,T ratios. A significant induction was found in the expression of Perforin and Granzyme B genes in eNKC relative to that of iNKC. Expression of mRNA was detected by Real Time PCR and normalized to GAPDH; (n=3)
