Table 7-5.

Predominantly CK7+/CK20−

TUMOR	CK7+ CK20+	CK7+ CK20–	CK7– CK20+	CK7– CK20–	34 β E12	CAM 5.2	CK 5/6	EMA	BER- EP4	CEA m	CEA p	TTF-1	P63	WT-1	S100	CHRO	HEP	OTHER
Acinic cell carcinoma	neg	POS	neg	neg	POS	POS		Mod			Low				POS	Low
Adenoid cystic carcinoma	neg	POS	neg	neg	POS	High	POS	Mod		POS	Low		POS		Mod	neg		GFAP Low
Breast ductal carcinoma	Low	High	neg	neg	nega	POS	Low	POS	High	High	Mod	neg	Lowa	High	Mod	Low	neg	ER/PRb GCDP Mod
Breast lobular carcinoma	Low	POS	neg	neg		POS	neg	POS	Mod	Mod	Mod	neg	Low			Low	neg	ER/PRb GCDP Mod E-cadherin neg
Brenner tumor	neg	POS	neg	neg				POS			High			Low	neg?	POS		Calretinin Low NSE POS
Cervical squamous cell carcinoma	neg	High	neg	Low	POS	neg	POS	POS		POS	Low	neg	POS			neg	neg	HPV POS p16 High
Choroid plexus	neg	High	neg	Low		POS		Low	neg						Mod			GFAP High
Chordoma	neg	POS	neg	neg	Mod	POS		POS	neg	neg	neg				POS	neg		GFAP neg
Craniopharyngioma	neg	POS	neg	neg	POS		POS
Embryonal carcinoma	neg	POS	neg	neg	neg	POS		Low		Low	Low	neg?	neg		neg	neg	neg	PLAP High CD30 High
Endometrial carcinoma	Low	High	neg	neg		POS	Low	POS	POS	Low	Low	neg		neg?	High	neg	neg	Vimentin POS ER High
Lung: adenocarcinoma	Low	High	neg	Low	Mod	POS	neg	POS	POS	High	High	High	High?	Low	Low	neg	Low
Lung: BALc non-mucinous	neg	POS	neg	neg	POS	POS				High	High	High	Mod	neg	Mod	neg
Meningioma: secretory typed	neg	POS	neg	neg		neg	High	POS		POS	POS				Low	neg		PR Mod ER neg
Mesothelioma	neg	High	neg	Low	High	POS	High	High	neg	neg	neg	neg	neg	High	neg	Low	neg	Calretinin High
Mixed tumore	neg	POS	neg	neg		POS	POS	Low		Low	neg?		POS		POS		neg	GFAP High SMA POS Calponin POS
Ovarian: endometrioid	neg	POS	neg	neg		POS	Low	POS	POS	Low	Low	neg?	Low	High	Low		neg	ER Mod
Ovarian:serous carcinoma	neg	POS	neg	neg		POS	Low	POS	POS	neg	neg	neg?	Low	POS	High		neg	ER High Calretinin Low
Renal cell: papillary and chromophobe	neg	POS	neg	neg	POS			POS						Modf
Thyroid:papillary	neg	POS	neg	neg	POS	POS	Mod	High		neg	Mod	POS	High		High	neg	neg	Thy POS Calci neg
Thyroid:follicular	neg	POS	neg	neg	neg		neg	Mod		neg	Low	POS			Mod	neg	neg	Thy POS Calci neg
Thyroid:medullary	neg	POS	neg	neg	neg		neg	neg		POS	Mod	POS				POS		Thy rare Calci POS

^ap63 may be positive in breast “basal like” carcinomas, some spindle cell metaplastic carcinomas, squamous cell carcinomas, and som e papillary carcinomas. These subtypes may also have less typical keratin subsets such as CK14 (detected by 34β E12), CK17 (detected by MNF-116), or CK5/6.

^bMost well and moderately differentiated ductal carcinomas, and carcinomas of special type (except for medullary) will be positive for hormone receptors. Poorly differentiated carcinomas, metaplastic carcinomas, and medullary carcinomas are usually negative. Well and moderately differentiated lobular carcinomas are almost always positive for ER, and usually positive for PR. Poorly differentiated lobular carcinomas may be negative for these markers.

^cNon-mucinous bronchiolo-alveolar carcinomas (BAL) have an immunophenotype similar to lung adenocarcinomas. Mucinous BALs are more likely to be CK20 positive (approximately 70% positive) and less likely to be TTF-1 positive (approximately 30% positive).

^dSecretory meningiomas are frequently positive for CK7 and CEA, whereas other subtypes are usually negative for CK7 and CEA. The majority of all types of meningiomas are positive for PR (including meningiomas in males).

^eMixed tumors (pleomorphic adenomas) occur most frequently in the salivary glands, but can also arise in soft tissues (myoepithelial tumors of soft tissue). These tumors have a similar immunophenotype with keratin (AE1/AE3 77%) or PANK (68%) or EMA (63%) present in the majority of tumors and frequent expression of markers associated with myoepithelial cells (e.g., calponin, GFAP, SMA, S100, p63). However, p63 is seen less frequently (23%) as compared to salivary tumors (100%).

^fChromophobe renal cell carcinomas may be positive for WT-1. Other types are negative.