Fig 5. Kidney-related parameters of beagles in the control group (closed circles; n = 8) and in the weight-change group (open circles; n = 8) during the weight-gain phase (week 0–47), weight-stable phase (week 47–56) and weight-loss phase (week 56–83).

Serum cystatin C (sCysC, A), a biomarker of kidney function; urinary protein:creatinine (UPC) (B), urinary immunoglobulin G:creatinine (uIgG/c, C), a biomarker of renal glomerular injury; and urinary neutrophil gelatinase-associated lipocalin:creatinine (uNGAL/c, D) a biomarker of renal tubular injury; urinary retinol-binding protein (uRBP/c, E) and time-to-peak (TTP) of the renal cortex, representing blood velocity, measured with contrast-enhanced ultrasound (F) are shown. ^aData from one dog missing in the control group. ***P = 0–0.001, **P = 0.001–0.01 and *P = 0.01–0.05 for the group x time interaction, after multiple correction.