Fig 3. Network-based Gene Ontology (GO) analysis and functional domain frequencies.

(A) Western blot validation of significantly up- and down-regulated proteins for Vimentin, Glial fibrillary acidic protein, Fragile X mental retardation syndrome-related protein 2, and Annexin a2. (B) Protein levels 0 and 60 min after reperfusion. (C) Network-based GO analysis yielded protein classification. Significantly down-regulated proteins (111 proteins) were mainly identified as organelle outer membrane proteins in the extracellular and plasma membrane regions whereas significantly up-regulated proteins (64 proteins) were found to be cytosolic ribosome proteins in the intracellular region or spliceosomal complex proteins in the intracellular and nucleus regions.