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. 2020 Apr 9;16(4):e1008675. doi: 10.1371/journal.pgen.1008675

Fig 2. Locust preferred detoxification genes and tryptamine catabolism genes to resist MAC infection.

Fig 2

(A) The detoxification genes of locusts were verified by qRT-PCR analysis. (B) qRT-PCR analyzed the gene expression of tryptamine catabolic enzyme in locusts after fungal infections. The results indicated that the gene expression of tryptamine Mao (LmMao-a and LmMao-b) and tryptamine hydroxylase cyp6j1 significantly increased in the fat bodies of MAC-infected locusts. However, (C) To observe the endogenous production of tryptamine of locust self, we performed the qRT-PCR analysis and found that the expression of the LmTdc gene for tryptamine production displayed no significant difference between pre- or post-infection of MAC. Thus, we speculate that the increase of tryptamine production mainly results from the fungus.(D) Given that KEGG enrichment showed that the detoxification pathways and tryptophan metabolism pathway of locusts was induced by MAC infection, we analyzed the tryptophan catabolism pathways in locusts. The arrow width indicates the main enzymes in insect tryptophan catabolism. All qPCR data were presented as mean ± standard error of the mean (SEM), Student’s t-test. ** indicated P < 0.01. n.s. indicated no significance.