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. 2020 Apr 9;16(4):e1008692. doi: 10.1371/journal.pgen.1008692

Fig 4. Deletion of Foxm1 from macrophages induces activation of p38 MAPK pathway.

Fig 4

(A-B) Interstitial and alveolar macrophages were isolated from bleomycin-treated myFoxm1-/- and control lungs using FACS sorting. qRT-PCR shows decreased Foxm1, and Dusp1 mRNAs and increased Tnf, Ccl5, Il1β, and Il6 mRNAs in flow-sorted interstitial and alveolar macrophages from bleomycin-treated myFoxm1-/- mice. Actb mRNA was used for normalization. N = 3 mice per group. (C) Increased expression of phosphorylated p38-MAPK (red) in MAC-3+ (green) macrophages was shown in bleomycin-treated myFoxm1-/- mice compared to control (upper panels). No difference in total p38-MAPK (red) was observed in macrophages in bleomycin-treated myFoxm1-/- mice compared to control (lower panels). (D) Co-localization studies showed decreased expression of DUSP1 (red) in MAC-3+ (green) macrophages in bleomycin-treated myFoxm1-/- mice. (E) Co-localization studies show increased expression of IL-1β (red) in MAC-3+ (green) macrophages in bleomycin-treated myFoxm1-/- mice. Scale bar = 50μm. (F) Percent of phospho-p38+/MAC3+, Total p38+/MAC3+, DUSP1+/MAC3+ and IL-1β+/MAC3+ double positive cells were counted in 5 random fields and presented as mean ± SEM. N = 3 mice per group. *P <0.05, **P <0.01, ***P <0.001 by Student’s t-test.