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(A) ChIP assay was performed to validate the binding with hnRNPA1 at the promoter of Pgc1; (B) ChIP-qPCR analysis of hnRNPA1 in Ad-GFP and Blnc1-over cells; (C)Western blot analysis of pre-adipocytes overexpression hnRNPA1; (D) The mRNA expression of hnRNPA1 and Pgc1β; (E1, E2) Proposed model for the Blnc1 mediated enhancement of Pgc1 transcription. ***p < 0.001, IgG vs. hnRNPA1 groups; #p < 0.05, Ad-GFP vs. Blnc1-over groups; ap < 0.05, cp < 0.01, over-nc vs. A1-over groups.
