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. 2020 Apr 17;13:3265–3276. doi: 10.2147/OTT.S220306

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© 2020 Shen et al.

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LncRNA-NEAT1 negatively regulated miR-124. (A) The expression of miR-124 in CC tissues and adjacent tissues was detected by qRT-PCR. (B) The expression of miR-124 in HaCaT, HeLa and SiHa cells was detected by qRT-PCR. (C) The correlation analysis between the expression of lncRNA-NEAT1 and miR-124 in CC tissues. (D) The binding target of miR-124 on lncRNA-NEAT1 was predicted by Target Scan. (E) The luciferase activity was detected by dual luciferase reporter gene assay. (F) The expression of miR-124 was detected by RNA pull-down assay. (G) The expression of miR-124 in transfected HeLa cells was detected by qRT-PCR. (H) The expression of miR-124 in transfected SiHa cells was detected by qRT-PCR. Data were presented as mean ± standard deviation with three replicates. **P < 0.01, ***P < 0.001, vs adjacent tissues (A), HaCaT cells (B), miR-NC group (E), Bio-NC group (F), and BLANK group (G and H).