Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo-sapiens) | hTERT RPE-1 | ATCC | CRL-4000 | Identity authenticated with STR Profiling by ATCC |
| Transfected construct (Homo-sapiens) | CEP83-Myc | Lo et al., 2019 | ||
| Antibody | FBF1 (rabbit polyclonal) | Proteintech, Rosemont, IL, USA | 11531–1-AP | 1/200 |
| Antibody | SCLT1 (rat polyclonal) | Tanos et al., 2013 | – | 1/250 |
| Antibody | CEP89 (rat polycloncal) | Tanos et al., 2013 | – | 1/500 |
| Antibody | ODF2-N (rabbit polyclonal) | Sigma-Aldrich | HPA001874 | 1/200 |
| Antibody | ODF2-C (rabbit polyclonal) | Abcam | ab43840 | 1/200 |
| Antibody | CEP128 (rabbit polyclonal) | Abcam | ab118797 | 1/200 |
| Antibody | CENTRIOLIN (mouse monoclonal) | Santa Cruz | sc-365521 | 1/200 |
| Antibody | NINEIN (rabbit polyclonal) | Bethyl | A301-504 | 1/1000 |
| Antibody | NINEIN (mouse monoclonal) | Santa Cruz | sc-376420 | 1/500 |
| Antibody | CEP170 (rabbit polyclonal) | Abcam | ab72505 | 1/400 |
| Antibody | CCDC68 | Proteintech | 26301–1-AP | 1/400 |
| Antibody | C-NAP1 | Santa Cruz | sc-390540 | 1/200 |
| Antibody | γ-tubulin | Sigma-Aldrich | T6557 | 1/500 |
| Antibody | α-tubulin | Santa Cruz | sc-32293 | 1/500 |
| Antibody | Centrin (mouse monoclonal) | Millipore | 04–1624 | 1/400 |
| Recombinant DNA reagent | gRNA cloning vector | Addgene | #41824 | |
| Recombinant DNA reagent |
CEP128 gRNA | (Mazo et al., 2016) | gRNA2 (5′-GCTGCCAGATCAACGCACAGGG-3′), gRNA4 (5′-GAGTCAGCTCTGAGATCTGAAGG-3′), gRNA5 (5′ GCAGCTGAACTTCAGCGCAATGG-3′) | |
| Recombinant DNA reagent | CEP83 gRNA | (Mazo et al., 2016) | gRNA1 (5′-GGTGGAGACAGTGGATTGACAGG-3′), gRNA2 (5′-GATATTAACTCCACAAAAATTGG-3′) | |
| Software, algorithm | Metamorph | Molecular Device | ||
| Software, algorithm | ImageJ | NIH |