Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| M. tuberculosis | H37Rv | Derived from E.R. Baldwin's human-lung isolate H37 by W. Steenken New York, United States, 1934 | (ATCC 25618) | |
| HIV-1 | ADA | Gift from Dr. S Benichou Institut Cochin, Paris, France |
N/A | |
| HIV-1 | NLAD8 | Gift from Dr. S Benichou Institut Cochin, Paris, France |
N/A | |
| HIV-1 | ADA Gag-iGFP-VSVG | This paper | This paper | |
| Buffy Coat | Leukocytes | Etablissement Français du Sang, Toulouse, France | N/A | |
| Lung biopsies from rhesus macaques | Histological slides | Tulane National Primate Research Center | N/A | |
| Cell line (human HeLa JC.53) | TZM-bl | NIH AIDS Reagent Program | Cat# 8129 | Cultured in: DMEM, 90%; FBS, 10%; 100 units of Penicillin and 0.1 mg/mL of Streptomycin |
| Cell line (human) | HEK-293T | NIH AIDS Reagent Program | Cat# 3318 | Cultured in: DMEM, 10% FCS |
| Cell line (human) | HEK-Blue IFN-α/β Cells | Invivogen | Cat# hkb-ifnab | Cultured in: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 µg/mL streptomycin, 100 µg/mL Normocin |
| Transfected construct (human) | siRNA to Siglec-1 (SMART-Pool) | Horizon Discovery | Cat# L-017521-01-0020 | (200 nM) |
| Transfected construct (human) | siRNA scramble (SMART Pool) | Horizon Discovery | Cat# D-001810-10-50 | (200 nM) |
| Antibody | Mouse monoclonal anti‑human Siglec-1 (clone 7-293) | Biolegend | Cat# 346008 RRID:AB_11147948 |
FACS (1 µg/mL) |
| Antibody | Mouse monoclonal anti‑human CD16 (clone 3G8) | Biolegend | Cat# 302019 and 302018; RRID:AB_492974 andAB_314218 | FACS (1 µg/mL) |
| Antibody | Mouse monoclonal anti‑human CD163 (clone GHI/61) | Biolegend | Cat# 333608 RRID:AB_2228986 |
FACS (1 µg/mL) |
| Antibody | Mouse monoclonal anti‑human MerTK (clone 590H11G1E3) | Biolegend | Cat# 367607 RRID:AB_2566400 |
FACS (1 µg/mL) |
| Antibody | Rabbit monoclonal anti‑human STAT1 (clone 42H3) | Cell Signaling Technology | Cat# 9175 RRID:AB_2197984 |
WB (1:100) |
| Antibody | Rabbit anti‑human actin (a.a. 20‑33) | Sigma‑Aldrich | Cat# A5060 RRID:AB_476738 |
WB (1:100) |
| Antibody | Rabbit polyclonal anti-a-tubulin | Abcam | Cat# ab18251 RRID:AB_2210057 |
IF (5 µg/mL) |
| Antibody | Mouse monoclonal anti-Siglec-1 (clone hsn 7D2) | Novus Biologicals | Cat# NB 600-534 RRID:AB_526814 |
IF (10 µg/mL) IHC (1:200) |
| Antibody | Mouse monoclonal anti-Gag RD1 (clone KC57) | NIH AIDS Reagent program | Cat# 13449 | IF (1:200) |
| Antibody | Mouse monoclonal anti-HIV-1 p24 (clone 183-H12-5C) | NIH AIDS Reagent Program | Cat# 3537 | ELISA (2.5 µg/mL) |
| Antibody | Human polyclonal anti-HIV Immune Globulin (HIVIG) | NIH AIDS Reagent Program | Cat# 3957 | ELISA (6.25 µg/mL) |
| Antibody | Polyclonal goat anti-human IgG | Sigma-Aldrich | Cat# A0170 | ELISA (1:10000) |
| Antibody | Mouse monoclonal anti‑human CD163 (clone 10D6) | Leica/Novocastra | Cat# NCL-L-CD163 RRID:AB_2756375 |
IHC (1:100) |
| Antibody | Anti-pSTAT1 | Cell Signaling Technology | Cat# 9167 RRID:AB_561284 |
WB (1:100) |
| Antibody | Mouse monoclonal anti-IFNAR2 (clone MMHAR-2) | Thermo Fisher Scientific | Cat# 213851 RRID:AB_223508 |
Blocking (20 µg/mL) FACS (1 µg/mL) |
| Antibody | Mouse IgG2a isotype control | Thermo Fisher Scientific | Cat# 02-6200 RRID:AB_2532943 |
Blocking (20 µg/mL) IF (0.6 µg/mL) |
| Antibody | Polyclonal F(ab)2 goat anti‑rabbit IgG, AlexaFluor 555 | Thermo Fisher Scientific | Cat# A-21430 RRID:AB_2535851 |
IF (2 µg/mL) |
| Antibody | Polyclonal F(ab)2 goat anti‑mouse IgG, AlexaFluor 488 | Thermo Fisher Scientific | Cat# A-10684 RRID:AB_2534064 |
IF (2 µg/mL) |
| Antibody | Plyclonal F(ab)2 goat anti‑mouse IgG, AlexaFluor 555 | Cell Signaling Technology | Cat# 4409 RRID:AB_1904022 |
IF (2 µg/mL) |
| Antibody | Polyclonal goat anti‑rabbit IgG, HRP | Thermo Fisher Scientific | Cat# 32460 RRID:AB_1185567 |
WB (1:10000) |
| Antibody | Polyclonal goat anti‑mouse IgG, HRP | Thermo Fisher Scientific | Cat# 31430 RRID:AB_228307 |
WB (1:10000) |
| Cytokine (recombinant, human) | M-CSF | Peprotech | Cat# 300‑25 | (20 ng/mL) |
| Cytokine (recombinant, human) | IFNb | Peprotech | Cat# 300-02BC | 10 and 100 U/mL |
| Cytokine (recombinant, human) | IL-10 | Peprotech | Cat# 200-10 | 10 ng/mL |
| Monocyte isolation | Mouse anti‑human CD14 microbeads | Miltenyi Biotec | Cat# 130‑050‑201 | |
| Monocyte isolation | LS magnetic columns | Miltenyi Biotec | Cat# 130‑042‑401 | |
| Western blot | Amersham ECL Prisme Western Blotting Detection Reagent | GE Healthcare | Cat# RPN2232 | |
| Western blot | SuperSignal WestPico Chemiluminescent Substrate | Thermo Scientific | Cat# 34080 | |
| ELISA | IL‑10 ELISA set | BD Bioscience | Cat# 555157 | |
| Cell culture | Trypsin EDTA 0.05% | Thermo Fisher Scientific | Cat# 25200072 | |
| Cell culture | Accutase | Sigma-Aldrich | Cat# A-6964 | |
| Probe | Phalloidin AlexaFluor 488 | Thermo Fisher Scientific | Cat# A12379 | (33 mM) |
| Probe | Phalloidin Alexa Fluor 647 | Thermo Fisher Scientific | Cat# A22287 | (33 mM) |
| Probe | DAPI | Sigma Aldrich | Cat# D9542 | (500 ng/mL) |
| Probe | CellTracker Green CMFDA Dye | Thermo Fisher Scientific | Cat# C7025 | (500 ng/mL) |
| Probe | MitoTracker Deep Red FM | Invitrogen | Cat# M22426 | (500 ng/mL) |
| IF | Fluorescence Mounting Medium | Agilent Technologies | Cat# S302380‑2 | |
| IF | Antibody diluent, Background reducing | DAKO, Agilent Technologies | Cat# S302283-2 | |
| Software | ImageJ | ImageJ | http://www.imagej.nih.gov/ij | |
| Software | Prism (v8.0.0) | GraphPad | http://www.graphpad.com | |
| Software | Photoshop CS3 | Adobe | http://www.adobe.com | |
| Software | Adobe Illustrator CS5 | Adobe | https://www.adobe.com/fr/products/illustrator.html | |
| Software | Huygens Professional Version 16.10 | Scientific Volume Imaging | https://svi.nl/HuygensProfessional | |
| Software | FACS DIVA | BD Bioscience | http://www.bdbiosciences.com/ | |
| Software | FlowJo_v10 | FlowJo | https://www.flowjo.com/ | |
| Software | FCS Express V3 | DeNovo Software | http://www.denovosoftware.com | |
| Software | Image Lab | Bio‑Rad Laboratories | http://www.bio‑rad.com | |
| Software | Pannoramic Viewer | 3DHISTECH | https://www.3dhistech.com/pannoramic_viewer |