Figure 8. Levels of Thyroid stimulating hormone in Astyanax mexicanus under different experimental conditions and from different populations.

(A) Mean Thyroid stimulating hormone levels normalized by protein concentration in adult surface fish (SF) and Pachón cavefish (PA) kept in D/D or L/D conditions for 1.5 to 2 years. N = 3 (SF L/D), 4 (SF D/D), 3 (PA L/D), 3 (PA D/D). (B) Mean thyroid stimulating hormone levels in SF (N = 8) and three different CF populations: Pachón (PA) (N = 5), Tinaja (TI) (N = 3) and Molino (MO) (N = 4) caves. (Error bars represent SD in three technical replicates. N ranges from 3 to 8 fish/group; *p<0.05; **p<0.01 as calculated by ANOVA and Tukey HSD Test. In B ns or * denotes significance in comparison to SF.) Figure 8—source data 1 contains raw data and summary statistics.