. 2020 Apr 21;9:e52701. doi: 10.7554/eLife.52701

Table 1. Complete genotypes of Drosophila strains used in this study.

Figure	Panel	Abbreviated genotype	COMPLETE GENOTYPE (‘Y’ represents the Y chromosome)
Figure 1	A, B	dsx^GAL4 ∩ fru^FLP ♂	w/Y; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022/+; dsx^GAL4/fru^FLP
Figure 1—figure supplement 1	A
Video 1
Figure 1	B	dsx^GAL4 ∩ fru^FLP ♀	w/w; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022/+; dsx^GAL4/fru^FLP
Figure 1—figure supplement 1	B	dsx^GAL4 ∩ fru^FLP ♀
Figure 1	E-I	dsx^GAL4, UAS > stop > CsChrimson, fru^FLP	w/Y; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022/+; dsx^GAL4/fru^FLP
Figure 1—figure supplement 1	C, D	dsx^GAL4, UAS > stop > CsChrimson, fru^FLP
Figure 1	E-H	dsx^GAL4, UAS > stop > CsChrimson	w/Y; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022/+; dsx^GAL4/+
Figure 1—figure supplement 1	C, D	dsx^GAL4, UAS > stop > CsChrimson
Figure 1	E-H	UAS > stop > CsChrimson, fru^FLP	w/Y; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022/+; fru^FLP/+
Figure 1—figure supplement 1	C, D	UAS > stop > CsChrimson, fru^FLP
Figure 1—figure supplement 2	B	Canton-S ♂	+/Y; +/+; +/+ (Canton-S)
Figure 1—figure supplement 2	B	Canton-S ♀	+/+; +/+; +/+ (Canton-S)
Figure 2	A, B	NP2631, UAS > stop > CsChrimson, dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/+
Figure 2—figure supplement 1	A-C
Figure 2—figure supplement 3	A, B
Video 2
Figure 2	A, B	NP2631, UAS > stop > CsChrimson	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; fru^4-40/+
Figure 2—figure supplement 3	A, B	NP2631, UAS > stop > CsChrimson
Figure 2	A, B	UAS > stop > CsChrimson, dsx^FLP	w/Y; +/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/+
Figure 2—figure supplement 3	A, B	UAS > stop > CsChrimson, dsx^FLP
Figure 2	C	NP2631, UAS > stop > CsChrimson, dsx^FLP, fru^{P1. LexA}, LexAop2-GAL80	w/Y; NP2631/8XLexAop2-GAL80 in attP40; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/fru^{P1. LexA}
Figure 2	C	NP2631, UAS > stop > CsChrimson, dsx^FLP, LexAop2-GAL80	w/Y; NP2631/8XLexAop2-GAL80 in attP40; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/+
Figure 2	C	NP2631, UAS > stop > CsChrimson, dsx^FLP, fru^{P1. LexA}	w/Y; NP2631/+; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/fru^{P1. LexA}
Figure 2—figure supplement 1	A-C	NP2631, UAS > stop > CsChrimson, dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/+
Figure 2—figure supplement 2	A-D	NP2631 ∩ dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP/+
Figure 2—figure supplement 2	E, H	NP2631, UAS > stop > CsChrimson (VK00005), dsx^FLP, fru^{P1. LexA}, LexAop2-GAL80	w/Y; NP2631/8XLexAop2-GAL80 in attP40; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/fru^{P1. LexA}
Figure 2—figure supplement 2	F, H	NP2631, UAS > stop > CsChrimson (VK00005), dsx^FLP, LexAop2-GAL80	w/Y; NP2631/8XLexAop2-GAL80 in attP40; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/+
Figure 2—figure supplement 2	G, H	NP2631, UAS > stop > CsChrimson (VK00005), dsx^FLP, fru^{P1. LexA}	w/Y; NP2631/+; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/fru^{P1. LexA}
Figure 3	C, G, H, L-N	NP2631 ∩ dsx^FLP, XY, fru locus: fru^M/fru^4-40	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^M
Figure 3—figure supplement 1	F
Video 3, 4
Figure 3	D, G, I, L-N	NP2631 ∩ dsx^FLP, XX, fru locus: fru^M/fru^4-40	w/w; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^M
Video 5		NP2631 ∩ dsx^FLP, XX, fru locus: fru^M/fru^4-40
Figure 3	E, G, J, L-N	NP2631 ∩ dsx^FLP, XY, fru locus: fru^F/fru^4-40	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^F
Video 4		NP2631 ∩ dsx^FLP, XY, fru locus: fru^F/fru^4-40
Figure 3	F, G, K-N	NP2631 ∩ dsx^FLP, XX, fru locus: fru^F/fru^4-40	w/w; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^F
Figure 3—figure supplement 1	H
Video 3, 5
Figure 3	G	NP2631 ∩ dsx^FLP, XY, fru locus: +/+	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP/+
Figure 3—figure supplement 1	A, E	NP2631 ∩ dsx^FLP, XY, fru locus: +/+
Figure 3	G	NP2631 ∩ dsx^FLP, XX, fru locus: +/+	w/w; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP/+
Figure 3—figure supplement 1	B, G	NP2631 ∩ dsx^FLP, XX, fru locus: +/+
Figure 3	G	NP2631 ∩ dsx^FLP, XY, fru locus: +/fru^4-40	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/+
Figure 3—figure supplement 1	C	NP2631 ∩ dsx^FLP, XY, fru locus: +/fru^4-40
Figure 3	G	NP2631 ∩ dsx^FLP, XX, fru locus: +/fru^4-40	w/w; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/+
Figure 3—figure supplement 1	D	NP2631 ∩ dsx^FLP, XX, fru locus: +/fru^4-40
Figure 3—figure supplement 1	I	NP2631, UAS > stop > CsChrimson, dsx^FLP, fru locus: +/+	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP/+
Figure 3—figure supplement 1	I	NP2631, UAS > stop > CsChrimson, dsx^FLP, fru locus: +/fru^4-40	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/+
Figure 3—figure supplement 1	I	NP2631, UAS > stop > CsChrimson, dsx^FLP, fru locus: fru^M/fru^4-40	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^M
Figure 4	A-C	fruF ♂, NP2631, UAS > stop > CsChrimson, dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^F
Figure 4—figure supplement 1	A, B
Video 6
Figure 4	A, B	fruF ♂, NP2631, UAS > stop > CsChrimson	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; fru^4-40/fru^F
Figure 4	A, B	fruF ♂, UAS > stop > CsChrimson, dsx^FLP	w/Y; +/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^F
Figure 4	C	♂, NP2631, UAS > stop > CsChrimson,dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^M
Figure 5	A, B	NP2631 ∩ dsx^FLP, R15A01-LexA	w/Y; NP2631/13XLexAop2-IVS-GCaMP6f-p10 in su(Hw)attP5; R15A01-LexA in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP
Figure 5	C, D	NP2631 ∩ dsx^FLP, R71G01-LexA	w/Y; NP2631/13XLexAop2-IVS-GCaMP6f-p10 in su(Hw)attP5; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP/R71G01-LexA in attP2
Figure 5	E, G	P1^a-GAL4	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2/+
Video 7		P1^a-GAL4
Figure 5	F, G	NP2631 ∩ dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP, fru^4-40/fru^M
Video 7		NP2631 ∩ dsx^FLP
Figure 5—figure supplement 1	A	P1^a-GAL4, UAS-CsChrimson	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2/+
Figure 5—figure supplement 1	A	P1^a-GAL4	w/Y; R15A01-p65AD:Zp in attP40/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2/+
Figure 5—figure supplement 1	A	UAS-CsChrimson	w/Y; +/20XUAS-IVS-CsChrimson:tdTomato in VK00022; +/+
Figure 5—figure supplement 1	B	P1^a-GAL4, UAS-CsChrimson	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^M
Figure 5—figure supplement 1	B	P1^a-GAL4	w/Y; R15A01-p65AD:Zp in attP40/10XUAS-IVS-GFP-p10 in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^M
Figure 5—figure supplement 1	B	UAS-CsChrimson	w/Y; +/20XUAS-IVS-CsChrimson:tdTomato in VK00022; fru^4-40/fru^M
Figure 5—figure supplement 1	C, D	P1^a-GAL4	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2/+
Figure 5—figure supplement 2	A	P1^a-GAL4, UAS-CsChrimson (VK00022)	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp;GAL4DBD in attP2/+
Figure 5—figure supplement 2	A	P1^a-GAL4, UAS > stop > CsChrimson (VK00022), fru^FLP	w/Y; R15A01-p65AD:Zp in attP40/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; R71G01-Zp;GAL4DBD in attP2/fru^FLP
Figure 5—figure supplement 2	A	P1^a-GAL4, UAS > stop > CsChrimson (VK00005), fru^FLP	w/Y; R15A01-p65AD:Zp in attP40/+; 71G01-Zp;GAL4DBD in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, fru^FLP
Figure 5—figure supplement 2	A	P1^a-GAL4, UAS > stop > CsChrimson (VK00005), dsx^FLP	w/Y; R15A01-p65AD:Zp in attP40/+; 71G01-Zp;GAL4DBD in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, dsx^FLP
Figure 5—figure supplement 2	C, E	P1^a-GAL4 ∩ fru^FLP	w/Y; R15A01-p65AD:Zp in attP40/+; 71G01-Zp;GAL4DBD in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, fru^FLP
Figure 5—figure supplement 2	D, E	NP2631 ∩ dsx^FLP	w/Y; NP2631/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00022; dsx^FLP/+
Figure 5—figure supplement 2	B	P1^a-GAL4,UAS > stop > CsChrimson, fru^FLP	w/Y; R15A01-p65AD:Zp in attP40/+;R71G01-Zp:GAL4DBD in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, fru^FLP
Figure 5—figure supplement 2	B	P1^a-GAL4, UAS > stop > CsChrimson	w/Y; R15A01-p65AD:Zp in attP40/+; R71G01-Zp;GaL4DBD in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005
Figure 5—figure supplement 2	B	UAS > stop > CsChrimson,fru^FLP	w/Y; R15A01-p65AD:Zp in attP40/+; +/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, fru^FLP
Figure 5—figure supplement 3	A, B	NP2631 ∩ fru^FLP, R15A01-LexA	w/Y; NP2631/13XLexAop2-IVS-GCaMP6f-p10 in su(Hw)attP5; R15A01-LexA in attP2/20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, fru^FLP
Figure 5—figure supplement 3	C, D	NP2631 ∩ fru^FLP, R71G01-LexA	w/Y; NP2631/13XLexAop2-IVS-GCaMP6f-p10 in su(Hw)attP5; 20XUAS > myr:TopHAT2 > CsChrimson:tdTomato in VK00005, fru^FLP/R71G01-LexA in attP2
Figure 6	A, E, F, H, I	P1^a-GAL4, XY, fru locus: fru^M/fru^4-40	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^M
Figure 6—figure supplement 1	F	P1^a-GAL4, XY, fru locus: fru^M/fru^4-40
Figure 6	B, E	P1^a-GAL4, XX, fru locus:fru^M/fru^4-40	w/w; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^M
Figure 6	C, E, G-I	P1^a-GAL4, XY, fru locus: fru^F/fru^4-40	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^F
Figure 6	D, E	P1^a-GAL4, XX, fru locus:fru^F/fru^4-40	w/w; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^F
Figure 6	E, H, I	P1^a-GAL4, XY, fru locus: +/+	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2/+
Figure 6—figure supplement 1	A, E	P1^a-GAL4, XY, fru locus: +/+
Figure 6	E	P1^a-GAL4, XX, fru locus: +/+	w/w; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2/+
Figure 6—figure supplement 1	B	P1^a-GAL4, XX, fru locus: +/+
Figure 6	E	P1^a-GAL4, XY, fru locus: +/fru^4-40	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/+
Figure 6—figure supplement 1	C	P1^a-GAL4, XY, fru locus: +/fru^4-40
Figure 6	E	P1^a-GAL4, XX, fru locus: +/fru^4-40	w/w; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/+
Figure 6—figure supplement 1	D	P1^a-GAL4, XX, fru locus: +/fru^4-40
Figure 6—figure supplement 1	G	P1^a-GAL4, UAS-CsChrimson, fru locus: +/+	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, +/+
Figure 6—figure supplement 1	G	P1^a-GAL4, UAS-CsChrimson, fru locus: +/fru^4-40	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/+
Figure 6—figure supplement 1	G	P1^a-GAL4, UAS-CsChrimson, fru locus: fru^M/fru^4-40	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^F
Figure 6—figure supplement 2	A-C	fruF ♂, P1^a-GAL4, UAS-CsChrimson	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^F
Figure 6—figure supplement 2	A	fruF ♂, P1^a-GAL4	w/Y; R15A01-p65AD:Zp in attP40/10XUAS-IVS-GFP-p10 in VK00022; R71G01-Zp:GAL4DBD in attP2, fru^4-40/fru^F
Figure 6—figure supplement 2	A	fruF ♂, UAS-CsChrimson	w/Y; R15A01-p65AD:Zp in attP40/20XUAS-IVS-CsChrimson:tdTomato in VK00022; fru^4-40/fru^F
Figure 6—figure supplement 2	C	♂, P1^a-GAL4, UAS-CsChrimson