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. 2020 Mar 26;8(1):e000172. doi: 10.1136/jitc-2019-000172

Figure 6.

Figure 6

Local growth and metastatization of B16.F0 cells in wild-type (WT) mice transduced with adeno-associated virus (AAV) encoding smCD6 protein. (A) C57BL/6J mice administered viral genome with 1011 AAV-smCD6 or AAV-Luc viral genome (v.g.)/mouse were challenged 2 weeks later with subcutaneous B16.F0 (5×104) cells. Tumor area (mm2) monitored twice a week is represented. (B) C57BL/6J mice administered intravenously with 1011 AAV-smCD6 or AAV-Luc v.g./mouse were challenged intravenously (tail vein) 2 weeks later with B16.F0 (2×105) cells. Number of lung metastasis and lung weight (GR) was determined 3 weeks after challenge. (C) Kaplan-Meier survival curve of an independent experiment performed under the same conditions as in (B). (D) C57BL/6J mice transduced intravenously with 108, 109, 1011 AAV-smCD6 or AAV-Luc v.g./mouse were challenged 2 weeks later with B16.F0 (2×105) cells intravenously injected in the tail. Number of lung metastasis determined 3 weeks after challenge is represented. All data represent cumulative results from two independent experiments expressed as mean±SEM. *p<0.05; **p<0.01; two-tailed Student’s t-test.