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. 2020 Apr 7;8(1):e000655. doi: 10.1136/jitc-2020-000655

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Changes in immune-cell subsets within prostatic tissue before and after PROSTVAC vaccine therapy. All cell densities were quantified as previously described using inForm software. (A) Frequency of T-regulatory cells (Tregs) (CD4+FOXP3+) before and after PROSTVAC therapy in non-compartmentalized analysis (NCA). (B) Frequency of T helper cells (CD4+FOXP3–) before and after PROSTVAC therapy in NCA. (C, D) Activated (Ki67+) and non-activated (Ki67–) CD8 cytotoxic T lymphocytes were quantified using NCA in both prevaccination and postvaccination sections. For (A–D), Wilcoxon signed rank test was used. Median±IQR is shown with horizontal lines. (E, F) Representative images of all four immune-cell subsets (Tregs, T helper, activated cytotoxic T lymphocytes and non-proliferative cytotoxic T lymphocytes).