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. 2020 Apr 7;8(1):e000655. doi: 10.1136/jitc-2020-000655

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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Antigen-specific T-cell responses assessed by intracellular cytokine staining of peripheral blood mononuclear cells following in vitro stimulation (pIR). Responses to the vaccine target antigen prostate-specific antigen and the cascade antigens MUC-1 and brachyury were measured by increases in the number of CD4+ or CD8+ lymphocytes producing cytokines (interferon-γ, tumor necrosis factor-α or interleukin-2) or positive for CD107a. An increase of ≥twofold from baseline was considered positive. pIR, peripheral immune response.