Table 1.
Number of revertant colonies after 3 mg/g hispidin‐enriched Sanghuangporus sanghuang mycelia exposure in the absence and presence of S9 mix
| Dose (µl/plate) | No. of histidine revertants/plate | ||||
|---|---|---|---|---|---|
| TA98 | TA100 | TA102 | TA1535 | TA1537 | |
| +S9 | |||||
| Spontaneous reversion rate | 42 ± 2 | 128 ± 15 | 251 ± 9 | 14 ± 2 | 15 ± 3 |
| S. sanghuang mycelia 0.3125 | 38 ± 3 | 137 ± 6 | 264 ± 15 | 14 ± 1 | 15 ± 3 |
| S. sanghuang mycelia 0.625 | 43 ± 3 | 128 ± 19 | 272 ± 23 | 16 ± 3 | 15 ± 4 |
| S. sanghuang mycelia 1.25 | 43 ± 6 | 135 ± 3 | 264 ± 21 | 18 ± 4 | 16 ± 2 |
| S. sanghuang mycelia 2.5 | 45 ± 4 | 136 ± 5 | 288 ± 22 | 17 ± 2 | 15 ± 3 |
| S. sanghuang mycelia 5 | 44 ± 2 | 137 ± 10 | 260 ± 25 | 17 ± 2 | 17 ± 2 |
| Positive controlsa | 334 ± 12b | 449 ± 28b | 598 ± 19b | 155 ± 10b | 397 ± 13b |
| −S9 | |||||
| Spontaneous reversion rate | 30 ± 2 | 137 ± 8 | 220 ± 24 | 20 ± 5 | 10 ± 2 |
| S. sanghuang mycelia 0.3125 | 23 ± 2 | 131 ± 4 | 204 ± 12 | 16 ± 2 | 12 ± 1 |
| S. sanghuang mycelia 0.625 | 28 ± 5 | 139 ± 6 | 209 ± 6 | 15 ± 2 | 12 ± 1 |
| S. sanghuang mycelia 1.25 | 29 ± 5 | 138 ± 5 | 202 ± 13 | 17 ± 3 | 12 ± 2 |
| S. sanghuang mycelia 2.5 | 31 ± 3 | 131 ± 3 | 205 ± 5 | 18 ± 3 | 14 ± 2 |
| S. sanghuang mycelia 5 | 30 ± 2 | 134 ± 7 | 202 ± 10 | 20 ± 2 | 13 ± 2 |
| Positive controlsc | 316 ± 20b | 970 ± 28b | 929 ± 34b | 779 ± 32b | 524 ± 9b |
Results are expressed as mean ± SD of triplicates.
a
TA98, TA102: Benzo[a]pyrene, 4 μg/plate; TA100: 2‐aminofluorene, 4 μg/plate; TA1535; TA1537: 2‐aminoanthracene, 4 μg/plate.
b
Twofold increase or more in revertant numbers over the negative control.
c
TA98: 4‐nitroquinoline‐N‐oxide, 0.5 μg/plate; TA100 and TA1535: sodium azide, 0.4 μg/plate; TA102: Mitomycin C, 0.5 μg/plate; TA1537: 9‐aminoacridine, 50 μg/plate.