Table 3.
Effect of McEO and their combination with nisin on the microbial load of aerobic plate count (APC), psychrotrophic count (PTC), and Enterobacteriaceae count (log10 CFU/g) of raw minced meat beef during storage at 4°C
| Days of storage at 4°C | |||||
|---|---|---|---|---|---|
| 0 | 3 | 7 | 14 | 21 | |
| APC | |||||
| C | 2.40 ± 0.15 | 4.30 ± 0.35 | 5.66 ± 0.20 | 6.95 ± 0.31 | 7.58 ± 0.07 |
| T1 | 2.40 ± 0.15 | 4.20 ± 0.28 | 5.60 ± 0.19 | 6.75 ± 0.20 | 6.90 ± 0.22 |
| T2 | 2.40 ± 0.15 | 4.15 ± 0.06 | 5.02 ± 0.08 | 6.44 ± 0.13 | 6.10 ± 0.18 |
| T3 | 2.40 ± 0.15 | 3.86 ± 0.19 | 4.70 ± 0.17 | 5.25 ± 0.26 | 5.60 ± 0.01 |
| T4 | 2.40 ± 0.15 | 3.28 ± 0.20 | 3.83 ± 0.22 | 4.90 ± 0.15 | 5.66 ± 0.10 |
| T5 | 2.40 ± 0.15 | 2.80 ± 0.17 | 3.00 ± 0.09 | 4.09 ± 0.12 | 5.25 ± 0.16 |
| PTC count | |||||
| C | 2.10 ± 0.12 | 4.86 ± 0.02 | 5.80 ± 0.05 | 7.20 ± 0.14 | 8.09 ± 0.19 |
| T1 | 2.10 ± 0.12 | 4.38 ± 0.02 | 5.49 ± 0.01 | 6.20 ± 0.6 | 6.85 ± 0.12 |
| T2 | 2.10 ± 0.12 | 4.37 ± 0.02 | 4.71 ± 0.02 | 5.33 ± 0.13 | 6.22 ± 0.24 |
| T3 | 2.10 ± 0.12 | 4.00 ± 0.03 | 4.61 ± 0.01 | 5.10 ± 0.12 | 5.94 ± 0.18 |
| T4 | 2.10 ± 0.12 | 2.66 ± 0.01 | 3.22 ± 0.17 | 4.20 ± 0.13 | 5.50 ± 0.19 |
| T5 | 2.10 ± 0.12 | 2.61 ± 0.02 | 3.00 ± 0.02 | 3.40 ± 0.13 | 5.09 ± 0.21 |
| Enterobacteriaceae count | |||||
| C | <1 | 2.30 ± 0.08 | 3.19 ± 0.01 | 3.80 ± 0.11 | 4.35 ± 0.08 |
| T1 | <1 | 2.00 ± 0.11 | 2.42 ± 0.14 | 3.60 ± 0.16 | 3.35 ± 0.18 |
| T2 | <1 | 1.70 ± 0.10 | 2.20v0.19 | 2.40 ± 0.11 | 3.20 ± 0.16 |
| T3 | <1 | 1.55 ± 0.11 | 1.88 ± 0.17 | 1.90 ± 0.15 | 2.20 ± 0.09 |
| T4 | <1 | 1.49 ± 0.11 | 1.62 ± 0.01 | 2.00 ± 0.11 | 2.09 ± 0.01 |
| T5 | <1 | <1 | <1 | 1.22 ± 0.12 | 1.66 ± 0.15 |
Values are given as mean ± SD of triplicate experiments.
(T0): control/untreated sample, (T1): treatment with McEO at 0.4% v/w; (T2): treatment with McEO at 0.8% v/w; (T3): treatment with nisin at 500 AU/g; (T4): treatment with McEO at 0.4% v/w + nisin at 500 AU/g; (T5): treatment with McEO at 0.8% v/w + nisin at 500 AU/g.