Table 3.
Growth parameters of H. elongata DSM 2581T (wildtype) and MS channel mutant MSC-1324
| [NaCl] | Growth rate [h−1] | Generation time [h] | Max OD600 | t (max OD600) [h] | ||||
|---|---|---|---|---|---|---|---|---|
| Wt | MSC-1324 | Wt | MSC-1324 | Wt | MSC-1324 | Wt | MSC-1324 | |
| 0.1 M | 0.22 | 0.26 | 3.09 | 2.71 | 1.04 | 1.04 | 32 | 38 |
| 1.0 M | 0.38 | 0.41 | 1.84 | 1.69 | 1.40 | 1.39 | 60 | 60 |
| 2.0 M | 0.24 | 0.30 | 2.88 | 2.33 | 1.28 | 1.29 | 48 | 48 |
Growth rate, generation time and maximal optical density at 600 nm (OD600) were compared. Both strains were grown in MM63 medium at low (0.1 M NaCl), medium (1 M NaCl), and high salt (2 M NaCl). Data presented are from three independent experiments (three biological replicates). Each experiment was measured 9 times (9 technical replicates)