Table 2.
Novel DNA repeat families of Onchocerca volvulus.
| Repeat family | Predicted occurrences | Primer set | Cq value ± SD | % Efficiency | R2 |
|---|---|---|---|---|---|
| O-150 | 46 | NA | 20.60 ± 0.03 | 95.1 | 0.991 |
| A | 970 | I | 26.18 ± 1.04 | 104.0 | 0.975 |
| II | 25.37 ± 0.74 | 97.1 | 0.994 | ||
| III | 24.95 ± 0.08 | 100.6 | 0.997 | ||
| B | 1,000 | I | 27.09 ± 0.04 | 110.9 | 0.992 |
| II | 28.27 ± 0.01 | NA | NA | ||
| III | 27.41 ± 0.02 | NA | NA | ||
| C | 565 | I | 34.00 ± 0.14 | NA | NA |
| II | 29.09 ± 0.08 | NA | NA | ||
| III | 28.815 ± 0.12 | NA | NA | ||
| D | 1,402 | I | 29.99 ± 0.10 | NA | NA |
| II | 29.18 ± 0.00 | NA | NA | ||
| III | 29.745 ± 0.71 | NA | NA | ||
| E | 823 | I | 32.69 ± 0.05 | NA | NA |
| II | 27.27 ± 0.09 | NA | NA | ||
| III | 29.08 ± 0.01 | NA | NA | ||
| F | 172 | I | NA | NA | NA |
| II | 32.15 ± 0.16 | NA | NA | ||
| III | 27.18 ± 0.05 | NA | NA | ||
| G | 252 | I | NA | NA | NA |
| II | 26.01 ± 0.11 | 106.1 | 0.996 | ||
| H | 100 | I | NA | NA | NA |
| II | NA | NA | NA | ||
| I | 102 | I | 32.95 ± 0.09 | NA | NA |
| II | 28.36 ± 0.24 | NA | NA | ||
| J | 196 | I | 28.29 ± 0.05 | NA | NA |
The Cq values and ± SD of all primer sets for O. volvulus repeat families. The efficiency and R2 of standard curves are shown for five assays that had a Cq value within 6.6 Cqs of the O-150 assay Cq value. Cq, quantification cycle; NA, not applicable; SD, standard deviation.