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. 2020 Apr 21;10:6754. doi: 10.1038/s41598-020-63741-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Airway inflammation in OVA-treated TetO-Wnt5a;SM22-rtTA mice. (A) Representative immunohistochemistry image (left) and the quantification (right) of eosinophil’s endogenous peroxidase activity, visualized with DAB, obtained from paraffin-embedded lung tissue sections of mouse airways exposed to repeated allergen (ovalbumin) challenge vs PBS-treated controls. (B) Immunohistochemistry staining as in (A) of a CD68 antibody staining, indicative of macrophages. n.d. = not detectable. One-way ANOVA followed by Tukey’s post hoc test. Group sizes are n = 8, 8, 5, 10 for the respective groups (left to right). Data is expressed at the mean ± SEM. **p < 0.01, ***p < 0.001.