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. 2020 Apr 21;10:6754. doi: 10.1038/s41598-020-63741-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytokine profiles in whole lung homogenates in OVA-treated TetO-Wnt5a;SM22-rtTA mice. (A/B/C) Relative gene expression (RT-qPCR, left panel) and protein levels (Luminex ELISA, right panel) of IL4 (A), IL5 (B) and IL13 (C) in whole lung homogenates of wild type (WT) and transgenic (Tg) mice exposed to repeated allergen (ovalbumin) challenge vs PBS-treated controls. *p < 0.05 **p < 0.01 ***p < 0.001 Kruskal-Wallis ANOVA on ranks followed by Dunn’s multiple comparisons test. (D) Relative total serum IgE levels in blood. n.d. = not detectable. One-way ANOVA followed by Tukey’s post hoc test. Group sizes are n = 8, 8, 5, 10 for the respective groups (left to right). Data is expressed at the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.