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. 2020 Apr 21;10:6705. doi: 10.1038/s41598-020-63626-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fermentation profile of one of the holding tanks. The holding tanks were filled up to 0.5 L i.e. 50% of the primary fermentation tank in a water batch at 32 °C and constant stirring. The vertical red dotted line indicates the timepoint at which the bottle was full.