Table 3.

Primers used in this study.

Name	Sequences (5′-3′)	Annealing Temp	Extension time	Cycle no.
PCR (gDNA amplification)
intron 1F exon 2R	cagtctagacagcagggcaa agtaggatgtgccccaggag	55 °C	1 min	35
exon 16F exon 20R	caaggactctagcacctgg gaatctcccaagtcctgtga	60 °C	3 min	34
intron 14F exon 15R	catgctgggtggctgagaa ctcctcgtgtgtactacggc	60 °C	1 min	28
intron 5F intron 8R	cagagccctccaagtgaaga ttccaggaccaggtgacatc	60 °C	1 min	30
exon 10F intron 12R	actgccttccccgacttca ggagctttctgggatgag	60 °C	5 min	34
RT-PCR (GAA transcript amplification)
exon 1F exon 5R	ggaaactgaggcacggagcg ggaccacatccatggcattgc	60 °C	2 min	34
exon 4F exon 10R	gtatatcacaggcctcgccg ctggtcatggaactcagcca	60 °C	2 min	34
exon 9F exon 17R	gggggttttcatcaccaacga ctgccaagggcctctactgg	60 °C	2 min	34
exon 16F exon 20R	caaggactctagcacctgg gaatctcccaagtcctgtga	60 °C	2 min	34
exon 4F exon 19R	gtatatcacaggcctcgccg gaagttggagacagggacacc	60 °C	3 min	35
qPCR
GAA transcript (exon 1-2), amplification exon 1F(q) exon 1-2R(q)	tgggaaagctgaggttgtcg tcctacaggcccgctcc	Refer to Methods
TBP transcript (exon 1-2), amplification exon 1-2F(q) exon 2R(q)	tctttgcagtgacccagcatcac cctagagcatctccagcacactct