Figure 4.

DBC1 KO mice show a failed collagen turnover and impaired vascular proliferation in response to ANGII-induced hypertension. (A) Representative images of aorta stained with Picrosirius red from Control or ANGII-treated WT and DBC1 KO mice. Collagen fibers are stained in red. White lines indicate adventitia (A) and intima-media (I + M). Scale bar: 40 µm. (B) Collagen fibers quantitation from the intima-media tunica (n = 3 for control mice and 4 for ANGII treated mice). One-way ANOVA with Bonferroni’s post hoc test for multiple comparisons was done. ** mean P < 0.01. (C) Quantitation of the intima-media tunica thickness in WT and DBC1 KO mice in Control and ANGII-treated conditions. One-way ANOVA with Bonferroni’s post hoc test for multiple comparisons was done. * and *** means P < 0.05, and 0.001, respectively. (D) Representative pictures of Bromodeoxyuridine (BrdU) incorporation in WT and DBC1 KO mice aortas in Control and ANGII-treated conditions. Arrows indicate BrdU positive cells (DAB staining). Slides were counterstained with Hematoxylin. Scale bar: 20 µm. (E) Quantitation of BrdU positive cells in the intima-media tunica of WT and DBC1 KO mice treated with ANGII. One-tailed Mann-Whitney U test was done (DBC1 KO median of frequency = 2.8%; WT median of frequency = 6.2%), U = 7. No positive signals were detected in control mice (n = 7 per group).* means P < 0.05.