Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 21;10:6741. doi: 10.1038/s41598-020-63778-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effects of ISO-1 on the mRNA and protein expression of MIF, NF-κB p65 and TNF-α. Western blot was obtained by using gel under uniform standard conditions and by cutting the film under uniform standard exposure conditions. The film was cut according to marker and the target strip was retained. Therefore, Fig. 5 shows the cut film. High contrast (overexposed) films are not used. mRNA expression of (A) MIF, (B) NF-κB p65 and (C) TNF-α were examined by real-time qPCR using RNA extracted from PANC-1 cells treated without or with indicated concentrations of ISO-1 for 24 hours. (D) Western blot analyses were conducted on protein lysates extracted from PANC-1 cells treated with indicated concentrations of ISO-1 for 24 hours. (E) Quantitative densitometric analysis of protein bands first normalized to GAPDH and then expressed as fold change relative to untreated controls. Graphs are expressed as the mean ± SD of each group from 3 independent experiments. *p < 0.05, and **p < 0.01 when compared with untreated controls.