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. 2020 Apr 21;10:6689. doi: 10.1038/s41598-020-63688-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NCS has no impact on weight gain and adiposity, and energy balance in high fat diet- induced obesity. NCS did not change the effects of high fat diet on body weight (A), solid food intake (B) and liquid intake (D), body composition (C), as well as activity and energy expenditure (E–H), compared to fructose and sucrose, while compared to CH group, all four high fat diet- fed groups demonstrated significant increased weight gain and decreased resting energy expenditure. (****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05 indicates statistical difference from comparison between CH group and high fat diet- fed groups).