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. 2020 Apr 21;10:6771. doi: 10.1038/s41598-020-63496-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M-MSCs/PRP gel significantly improved the formation of new bone tissues. (A,B) Compared with blank control group, tetracycline fluorescence signal was not significantly enhanced in PRP group until 90 d; both M-MSCs and M-MSCs/PRP group displayed apparent tetracycline fluorescence signal. Compared with M-MSCs single group, M-MSCs/PRP group displayed stronger tetracycline fluorescence signal. Scale bar = 100 μm. (C,D), The new mineralized bone was labeled “red” by Gomori staining. Compared with blank control group, M-MSCs group or PRP group, the new mineralized bone area was significantly increased in M-MSCs/PRP group at 90 d. Scale bar = 100 μm. 3 randomized fields of view for each group, *P < 0.05, **P < 0.01.